Rotavirus Antigen Rapid Test
MAU OYAMBA
Matenda a Rotavirus ndi omwe amatsogolera pachimake m'mimba, makamaka mwa ana ang'onoang'ono.Kupezeka kwake mu 1973 ndi kuyanjana kwake ndi infantile gastro-enteritis kunayimira kupita patsogolo kofunika kwambiri pa kafukufuku wa gastroenteritis osati chifukwa cha matenda owopsa a bakiteriya.Rotavirus imafalikira ndi njira ya mkamwa ndi ndowe ndi makulitsidwe kwa masiku 1-3.Ngakhale kuti zitsanzo zomwe zasonkhanitsidwa mkati mwa tsiku lachiwiri ndi lachisanu la matendawa ndi zabwino kuti zizindikire ma antigen, rotavirus ikhoza kupezekabe pamene kutsekula m'mimba kumapitirira.Rotaviral gastroenteritis imatha kufa kwa anthu omwe ali pachiwopsezo monga makanda, okalamba ndi odwala omwe alibe chitetezo chamthupi.M'madera otentha, matenda a rotavirus amapezeka makamaka m'miyezi yozizira.Akuti miliri komanso miliri yokhudza anthu pafupifupi 1,000.Ndi ana ogonekedwa m'chipatala omwe akudwala matenda owopsa a enteric, mpaka 50% ya zitsanzo zomwe zidawunikidwa zinali zabwino za rotavirus.Ma virus amachulukana mu
cell nucleus ndipo imakonda kukhala mitundu yokhazikika yomwe imapanga mawonekedwe a cytopathic effect (CPE).Chifukwa rotavirus ndizovuta kwambiri ku chikhalidwe, sizodabwitsa kugwiritsa ntchito kudzipatula kwa kachilomboka pozindikira matenda.M'malo mwake, njira zosiyanasiyana zapangidwa kuti zizindikire rotavirus mu ndowe.
MFUNDO
Rotavirus Rapid Test Device (Nyenyewe) imazindikira rotavirus kudzera mu kumasulira kowoneka bwino kwamtundu wamkati.Ma antibodies a rotavirus sasunthika pagawo loyesa la nembanemba.Pa kuyeza, chitsanzo
imakhudzidwa ndi ma antibodies olimbana ndi rotavirus olumikizidwa ku tinthu tating'ono tamitundu ndipo amapaka kale papepala lachitsanzo la mayeso.The osakaniza ndiye migrates kudzera nembanemba ndi capillary kanthu ndi interacts ndi reagents pa nembanemba.Ngati alipo
rotavirus yokwanira pachitsanzocho, gulu lachikuda lidzapanga pagawo loyesa la nembanemba.Kukhalapo kwa gulu lachikuda ichi kumasonyeza zotsatira zabwino, pamene kusowa kwake kumasonyeza zotsatira zoipa.Kuwonekera kwa gulu lakuda pa
dera lowongolera limagwira ntchito ngati njira yoyendetsera, kuwonetsa kuti kuchuluka koyenera kwa chitsanzo chawonjezedwa ndikuwotcha kwa membrane kwachitika.
ZAMBIRI ZA KIT
| Zipangizo zoyeserera pawokha | Chida chilichonse chimakhala ndi mzere wokhala ndi ma conjugate amitundu ndi ma reagents okhazikika omwe amakutidwa kale m'magawo ofananirako. |
| Zitsanzo dilution chubu ndi buffer | 0.1 M Phosphate buffered saline (PBS) ndi 0.02% sodium azide. |
| Ma pipette otayika | Kutolera zitsanzo zamadzimadzi |
| Ikani phukusi | Pakuti malangizo ntchito |
ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
| Chowerengera nthawi | Kuti mugwiritse ntchito nthawi |
| Centrifuge | Zochizira zitsanzo zapadera zochitika |
Zitsimikizo
