ZOYENERA KUGWIRITSA NTCHITO
The StrongStep^®Adenovirus Rapid Test Device (Nyenyewe) ndiwowoneka mwachanguimmunoassay kwa qualitative presumptive kuzindikira adenovirus mwa anthuzitsanzo za ndowe.Izi zidapangidwa kuti zigwiritsidwe ntchito ngati chithandizo cha matenda a adenovirus
matenda.

MAU OYAMBA
Enteric adenoviruses, makamaka Ad40 ndi Ad41, ndizomwe zimayambitsa matenda otsekula m'mimba.mwa ana ambiri akudwala matenda otsekula m'mimba, chachiwirimatenda a rotavirus okha.Matenda otsekula m'mimba ndi omwe amachititsa imfamwa ana ang'onoang'ono padziko lonse lapansi, makamaka m'mayiko omwe akutukuka kumene.Adenovirustizilombo toyambitsa matenda tasiyanitsidwa padziko lonse lapansi, ndipo titha kuyambitsa kutsekula m'mimbamwa ana chaka chonse.Matendawa amapezeka kawirikawiri mwa ana ocheperapozaka ziwiri, koma zapezeka mwa odwala azaka zonse.Kafukufuku amasonyeza kuti adenoviruses amagwirizanitsidwa ndi 4-15% ya onsem'chipatala milandu ya viral gastroenteritis.

Kuzindikira mwachangu komanso molondola za gastroenteritis yokhudzana ndi adenovirus ndikothandizapakukhazikitsa etiology ya gastroenteritis ndi kasamalidwe ka odwala.Njira zina zodziwira matenda monga electron microscopy (EM) ndinucleic acid hybridization ndi yokwera mtengo komanso yogwira ntchito.Pozindikira zakudziletsa kudziletsa chikhalidwe cha matenda adenovirus, okwera mtengo ndikuyezetsa kolemetsa kwambiri sikungakhale kofunikira.

MFUNDO
Chipangizo cha Adenovirus Rapid Test Device (Nyenyewe) chimazindikira adenoviruskupyolera mu kutanthauzira kowoneka kwa chitukuko cha mtundu mkativula.Ma antibodies a anti-adenovirus sasunthika pagawo loyesamembrane.Pakuyesa, chitsanzocho chimagwira ndi ma antibodies a anti-adenoviruskulumikizidwa ku tinthu tating'ono tamitundu ndikuyika kale papepala lachiyeso.Kusakaniza kumasuntha kudzera mu nembanemba ndi capillary action ndikulumikizanandi ma reagents pa nembanemba.Ngati pali adenovirus yokwanira pachitsanzo, agulu lamitundu lidzapanga pagawo loyesa la nembanemba.Kukhalapo kwa izigulu lakuda likuwonetsa zotsatira zabwino, pomwe kusapezeka kwake kukuwonetsa zoyipazotsatira.Mawonekedwe a gulu lachikuda pa dera lolamulira amakhala ngati akuwongolera njira, kusonyeza kuti kuchuluka koyenera kwa chitsanzo kwachitikakuwonjezeredwa ndipo kupukuta kwa membrane kwachitika.

NTCHITO
Bweretsani zoyesa, zoyeserera, zotchingira ndi/kapena zowongolera kutentha kwachipinda(15-30 ° C) musanagwiritse ntchito.
1. Kutolereni zitsanzo ndi kuchiza:
1) Gwiritsani ntchito zotengera zaukhondo, zowuma potengera zitsanzo.Zotsatira zabwino zidzakhalazopezeka ngati kuyesako kuchitidwa mkati mwa maola 6 mutatolera.
2) Pazitsanzo zolimba: Chotsani ndi kuchotsa dilution chubu applicator.Khalanisamalani kuti musatayike kapena kuwaza madzi kuchokera muchubu.Sungani zitsanzopolowetsa ndodo yofunsira m'malo osachepera atatu osiyanasiyanaKutolera ndowe pafupifupi 50 mg ya ndowe (yofanana ndi 1/4 ya nandolo).Kwa zitsanzo zamadzimadzi: Gwirani pipette molunjika, aspirate fecalzitsanzo, ndiyeno kusamutsa madontho awiri (pafupifupi 80 µL) muchubu chosonkhanitsira zitsanzo chokhala ndi buffer yochotsa.
3) Bwezerani chogwiritsira ntchito mu chubu ndikupukuta kapu mwamphamvu.Khalanisamalani kuti musathyole nsonga ya chubu cha dilution.
4) Gwirani mwamphamvu chubu chosonkhanitsira chitsanzo kuti musakanize chitsanzocho ndibuffer yochotsa.Zitsanzo zokonzedwa mu chubu chotolera zitsanzoikhoza kusungidwa kwa miyezi 6 pa -20 ° C ngati siinayesedwe mkati mwa ola limodzi pambuyo pakekukonzekera.

2. Kuyesedwa
1) Chotsani kuyesa m'thumba lake losindikizidwa, ndikuyikapomalo oyera, osalala.Lembani mayesowo ndi wodwala kapena wowongolerachizindikiritso.Kuti mupeze zotsatira zabwino, kuyesedwa kuyenera kuchitidwa mkati mwa chimodziola.
2) Pogwiritsa ntchito kapepala kakang'ono, thyola nsonga ya chubu cha dilution.Gwiranichubu molunjika ndikugawa madontho atatu a yankho mumtsuko(S) ya chipangizo choyesera.Pewani kutchera thovu la mpweya pachitsanzo bwino (S), ndipo osawonjezera
njira iliyonse chifukwa zenera.Mayeso akayamba kugwira ntchito, mtundu umasuntha kudutsa nembanemba.

3. Dikirani kuti magulu achikuda awonekere.Zotsatira ziyenera kuwerengedwa pa 10mphindi.Osamasulira zotsatira pakatha mphindi 20.

Zindikirani:Ngati chitsanzocho sichimasamuka chifukwa cha kukhalapo kwa tinthu tating'onoting'ono, centrifugezitsanzo zochotsedwa zomwe zili mu vial yochotsamo.Sungani 100 µL wachapamwamba, perekani mu chitsime cha chitsanzo (S) cha chipangizo chatsopano choyesera ndikuyambanso, kutsatira malangizo omwe afotokozedwa pamwambapa.

Zitsimikizo