Kuyeza Kuyeza kwa Cervical Pre-cancer ndi Cancer
ZOYENERA KUGWIRITSA NTCHITO
The StrongStep®HPV 16/18 Antigen Rapid Test Device ndi njira yoyesera yodziwira mwachangu ya HPV 16/18 E6&E7 oncoproteins m'zitsanzo za khomo lachiberekero la akazi.Chidachi chimapangidwa kuti chigwiritsidwe ntchito ngati chithandizo pakuzindikira matenda a Cervical Pre-cancer ndi Cancer.
MAU OYAMBA
M'mayiko omwe akutukuka kumene, khansa ya pachibelekero ndi yomwe imayambitsa imfa ya amayi chifukwa cha kusakhazikika kwa zoyezetsa zoyezetsa khansa ya pachibelekero ndi khansa.Kuyesa kowunika kwa zoikamo zocheperako kuyenera kukhala kosavuta, kofulumira, komanso kogwira ntchito.Momwemo, kuyesa koteroko kungakhale kodziwitsa za HPV oncogenic zochita.Kufotokozera kwa HPV E6 ndi E7 oncoprotein ndikofunikira kuti kusintha kwa khomo lachiberekero kuchitike.Zotsatira zina za kafukufuku zinawonetsa kulumikizana kwa E6 & E7 oncoprotein positivity ndi kuopsa kwa khomo lachiberekero komanso chiopsezo chopita patsogolo.Chifukwa chake, E6&E7 oncoprotein imalonjeza kukhala chizindikiro choyenera cha zochitika za HPV-mediated oncogenic.
MFUNDO
The StrongStep®HPV 16/18 Antigen Rapid Test Device idapangidwa kuti izizindikira HPV 16/18 E6&E7 Oncoproteins kudzera mu kutanthauzira kowoneka kwa kakulidwe kamitundu mumzere wamkati.Nembanembayo inali yosasunthika ndi ma antibodies a monoclonal anti-HPV 16/18 E6&E7 pagawo loyesa.Pakuyesa, chitsanzocho chimaloledwa kuchitapo kanthu ndi ma anti-HPV 16/18 E6&E7 ma antibodies amtundu wamitundu yama conjugates, omwe adayikidwa kale pachitsanzo cha mayeso.Kusakaniza ndiye kumayenda pa nembanemba ndi capillary kanthu, ndi kucheza ndi reagents pa nembanemba.Ngati panali HPV 16/18 E6&E7 oncoprotein yokwanira m'zitsanzo, gulu lamitundu lidzapanga pagawo loyesa la nembanemba.Kukhalapo kwa gulu lachikudali kumasonyeza zotsatira zabwino, pamene kusowa kwake kumasonyeza zotsatira zoipa.Kuwonekera kwa gulu lachikuda pachigawo cholamulira kumagwira ntchito ngati njira yoyendetsera.Izi zikuwonetsa kuti kuchuluka koyenera kwa sampuli kwawonjezeredwa ndipo kupukuta kwa membrane kwachitika.
KUSONGA ZINTHU NDI KUSINTHA
■ Ubwino wa zitsanzo zomwe zapezedwa ndizofunika kwambiri.Momwemokhomo lachiberekero epithelial selo ayenera kusonkhanitsidwa ndi swab.Kwa zitsanzo za khomo lachiberekero:
■ Gwiritsani ntchito zida za Dacron kapena Rayon zokhazokha zokhala ndi zitsulo zapulasitiki.Zili chonchoamalimbikitsa kugwiritsa ntchito swab yoperekedwa ndi wopanga zida (The swab arezomwe zili mu kitchi, kuti mudziwe zambiri, chonde lemberani akupanga kapena wogawa wakomweko, nambala yamakasitomala ndi 207000).Swabskuchokera kwa ogulitsa ena sizinatsimikizidwe.Swabs ndi nsonga za thonje kapenamatabwa a matabwa osavomerezeka.
■ Musanasonkhanitse zitsanzo, chotsani ntchofu zochulukira ku khomo lachiberekerondi swab yosiyana kapena mpira wa thonje ndikutaya.Ikani swab mukhomo pachibelekeropo mpaka ulusi wakumunsi wokhawo utawonekera.Molimba tembenuzani swabkwa masekondi 15-20 mbali imodzi.Kokani swab mosamala!
■ Osayika swab mu chotengera chilichonse chomwe chili ndi sing'anga kuyambira pamenepozoyendera sing'anga kusokoneza assay ndi viability wa zamoyo ndisikufunika kuyesedwa.Ikani swab ku chubu chochotsa, ngati mayesoakhoza kuthamanga nthawi yomweyo.Ngati kuyezetsa mwamsanga sikutheka, wodwalayozitsanzo ziyenera kuikidwa mu chubu chowuma chonyamulira kuti zisungidwe kapena zonyamulira.Theswabs akhoza kusungidwa kwa maola 24 kutentha kwa firiji (15-30 ° C) kapena 1 sabatapa 4°C kapena osapitirira miyezi 6 pa -20°C.Zitsanzo zonse ziyenera kuloledwakufika kutentha kwa 15-30 ° C musanayese.
