Chidaliro Chachangu
Kugwiritsa Ntchito
Nyemba®Mayeso achikondi ndi omwe amadziwika kuti amadziwika kuti amadziwika kuti amadziwika kuti amazindikira igbbp-1 kuchokera ku mankhwalawa amtundu wamadzimadzi pa mimba. Kuyesedwa kumapangidwira ntchito ya akatswiri kuthandiza kuti adziwe kupasuka kwa fetal membrane (Rom) mwa amayi apakati.
Chiyambi
Kukhazikika kwa igfbp-1 (insulin-ngati proctor protein-1) mu Aminific madzi nthawi 100 mpaka 1000 kuposa ku seramu. IGFBP-1 Nthawi zambiri sizili ku nyini, koma zitayamba kuphwanya fetil membrane, mankhwalawa amtundu wakuda kwambiri wa igbb-1 sakaniza zinsinsi za ukazi. Mu mayeso amphamvu amphamvu, katswiri wa katulutsidwe wa ukazi amatengedwa ndi Swabter Swab ndipo fanizo limatengedwa mu yankho la m'gulu. Kukhalapo kwa igfbp-1 mu yankho kumadziwika pogwiritsa ntchito chipangizo choyesera mwachangu.
Maganizo
Nyemba®Mayeso a Pro Love amagwiritsa ntchito utoto immunoromatographict, ma capillary oyenda. Njira yoyesera imafuna kusungunuka kwa igbbp-1 kuchokera ku swab ya swab posakaniza swab pompopompo. Kenako buffer yosakanikirana imawonjezeredwa ku Cassette yamayeso yoyeserera bwino ndipo osakaniza amasamukira kukhoma. Ngati igbbp-1 ilipo mu chitsanzo, chidzapanga zovuta ndi antibod otsutsa-1 antibody olumikizidwa ndi tinthu tating'onoting'ono. Mavuto amphamvu adzamangidwa ndi antibod-1 antibody wokutidwa ndi nitrocellulose nembanemba. Maonekedwe a mzere woyeserera wowoneka komanso mzere wowongolera uziwonetsa zotsatira zabwino.
Zigawo zikuluzikulu
| 20 Payekha pankhaZida za Ed | Chipangizo chilichonse chimakhala ndi chingwe chokhala ndi conjuban conjugunk komanso zopangidwa ndi zisanachitike pamalo ofanana. |
| 2KutulutsaBuffer vial | 0.1 m phosphate yolumikizidwa saline (PBS) ndi 0.02% sodium azide. |
| 1 yodzitchinjiriza swab (pofunsira) | Khalani ndi igfbp-1 ndi sodium azide. Chifukwa chowongolera zakunja. |
| 1 zoyipa swab (pofunsira) | Osakhala ndi igfbp-1. Chifukwa chowongolera zakunja. |
| 20 Chigawo cha madzi | Zogwiritsa ntchito zowerengera. |
| 1 Kumanga | Ikani ndodo yolumikizira ndi machubu. |
| 1 Kuyika kwa phukusi | Kulangizidwa. |
Zida zofunika koma sizinaperekedwe
| Ikanthawi | Kugwiritsa ntchito nthawi. |
KUSAMALITSA
■ kwa akatswiri mu diactro wozindikira pokhapokha.
Osagwiritsa ntchito tsiku lotha ntchito lomwe lawonetsedwa pa phukusi. Osagwiritsa ntchito mayeso ngati thumba lake lowonongeka lawonongeka. Osagwiritsanso ntchito mayeso.
■ Towa ili ndi zinthu za zoyambira zanyama. Chidziwitso chotsimikizika cha chiyambi ndi / kapena chimbudzi cha nyama sizitanthauza kuti kusakhalapo kwa othandizira pathogenic. Chifukwa chake, ndikulimbikitsidwa kuti zinthu izi zimathandizidwa ngati mwazotheka, ndikuzisamalira mosamala (sizikuphatikiza kapena zotupa).
Musapewe kuipitsidwa ndi zoyeserera za zoyeserera pogwiritsa ntchito chidebe chatsopano cha fanizo lililonse.
Werengani njira yonse musanayambe kuyesedwa kulikonse.
Osamadya, kumwa kapena kusuta m'dera lomwe zoyerekeza ndi ma kits zimayendetsedwa. Samalani zonena zawo ngati ali ndi opatsirana opatsirana. Kuchita mosamala motsutsana ndi zoopsa zomwe zimachitika munthawi yonseyi ndikutsatira njira zoyenera zomwe zimatheka. Valani zovala zoteteza monga malaya a labotale, magolovesi otayika ndi kutetezedwa ndi malingaliro pomwe malingaliro aja aja.
Osasokoneza kapena kusakaniza regents kuchokera ku maenje osiyanasiyana. Osasakaniza yankho la botolo.
Chinyezi ndi kutentha zimatha kuvuta kwambiri.
Nthawi yomwe bungwe lagamulo limamalizidwa, pezani ma swab mosamala atatha kuwaletsetsa pa 121 ° C kwa mphindi 20. Kapenanso, amatha kuthandizidwa ndi 0,5% sodium hypochloride (kapena bulichi) kwa ola limodzi atatayidwa. Zipangizo zoyeserera zomwe zogwiritsidwa ntchito zimayenera kutayidwa molingana ndi kwanuko, boma ndi / kapena feduro.
Musagwiritse ntchito mabungwe a cytology ndi odwala apakati.
Kusunga ndi kukhazikika
■ Kitt iyenera kusungidwa pa 2-30 ° C mpaka tsiku lomaliza losindikizidwa ku thumba losindikizidwa.
■ Mayeso ayenera kukhala mu thumba losindikizidwa mpaka kugwiritsira ntchito.
Osasintha.
Mayankho ayenera kutengedwa kuti ateteze zinthu zomwe zimapezeka mu zoipitsidwa. Osagwiritsa ntchito ngati pali umboni wa kuipitsidwa kapena mpweya wabwino. Kuipitsa kwachilengedwe kwa kufalitsa zida, zotengera kapena zoyeserera zimatha kubweretsa zotsatira zabodza.
Kutolera mwachidule ndi kusungidwa
Gwiritsani ntchito ndalama zochepa kapena rayn zidagunda swab shabs ndi ma pulasitiki. Ndikulimbikitsa kugwiritsa ntchito swab yomwe imapereka ndi opanga (Swabs sanakhalepo pachiwonetserochi, kuti mudziwe zambiri, chonde lemberani wopanga kapena kugulitsa kwapafupi, nambala ya cataloge ndi 207000). Ma swabs ochokera kwa othandizira ena sanavomerezedwe. Swabs ndi upangiri wa thonje kapena ma shargets a matabwa sakulimbikitsidwa.
Nthawi imapezeka pogwiritsa ntchito shaweleter swab. Sampleyo iyenera kusonkhanitsidwa musanayambe kuyesedwa digito ndi / kapena transvaginal ultrasound. Samalani kuti musakhudze chilichonse ndi swab musanayambe kusamalira. Ikani mosamala nsonga ya swab kulowa ndi nyini kupita ku n'kumapita kumodzi mpaka kukwaniritsidwa. Kapenanso kuti sample itha kuchotsedwa pakhomo la Kterter Pannix panthawi yoyesedwa yopumira. Swab iyenera kusiyidwa mu nyini kwa masekondi 10-15 kuti ilole kuti ichotse pena pake. Kokani swab mosamala!.
■ Ikani swab kupita ku chubu cha madzi, ngati mayesowo atha kuthamanga nthawi yomweyo. Ngati kuyezetsa kumeneku sikungatheke, zitsanzo zodwala ziyenera kuyikidwa mu chubu chowuma chosungira kapena kunyamula. Masabata amatha kusungidwa kwa maola 24 firiji (15-30 ° C) kapena sabata 1 pa 4 ° pa -20 ° CO. Zojambula zonse ziyenera kuloledwa kufikira kutentha kwa chipinda cha 15-30 ° C musanayesedwe.
Machitidwe
Bweretsani mayesero, zoyerekeza, buffer ndi / kapena kuwongolera kutentha kwa chipinda (15-30 ° C) musanagwiritse ntchito.
Ikani chubu choyera choyera m'malo ophatikizidwa. Onjezani 1ML ya kuchotsera kubowola ku chubu cha madzi.
Ikani spimen swab mu chubu. Sakanizani mwamphamvu yankho pozungulira swab mwamphamvu kumbali ya chubu katatu (pomwe kumamizidwa). Zotsatira zabwino zimapezeka pomwe fanizoli likusakanizidwa mwamphamvu mu yankho.
Werengani madzi ambiri momwe mungathere kuchokera ku swab poyang'ana mbali ya chubu chosinthika monga swab imachotsedwa. Osachepera 1/2 mwa zitsanzo zothetsera buffer ziyenera kukhalabe mu chubu chosamukira ku capillary chokwanira kuti chichitike. Ikani chipewacho pa chubu chotuluka.
Kutaya swab mu chidebe choyenera cha bioharkous.
■ Zosintha zomwe zimachotsedwa zimatha kusunga kutentha kwa mphindi 60 osakhudza chifukwa cha mayeso.
Kuchotsa mayesowo m'thumba lake losindikizidwa, ndikuyika pa oyera pamtunda. Lembani chipangizocho ndi chizindikiritso kapena chowongolera. Kuti mupeze zotsatira zabwino, chigamulo chikuyenera kuchitidwa mkati mwa ola limodzi.
Onjezani madontho atatu (pafupifupi 100 μl) ya zitsanzo zosinthidwa kuchokera ku chubu cha madzi opita ku Samsette yoyeserera.
Pewani kutchera ma thovu a mpweya mu chinsalu (s), ndipo musataye yankho lililonse pazenera.
Mayeso akayamba kugwira ntchito, mudzaona mtunduwo ukusunthira kudutsa nembanemba.
■ Yembekezerani gulu lazithunzi. Zotsatira zake ziyenera kuwerengedwa kwa mphindi 5. Osatanthauzira zotsatira pambuyo pa mphindi 5.
Tayani machubu oyeserera ndi ma cassette oyenera mu chidebe choyenera cha bioankhoous.
Ntergretis ya zotsatira
| WosaipidwaMalipiro:
| Magulu awiri achikuda amawonekera pa nembanemba. Bandi imodzi imawonekera kudera la Control (c) ndi gulu lina limapezeka m'chigawo (T). |
| WosavomelaMalipiro:
| Gulu limodzi lokha lamtundu limapezeka m'chigawo chowongolera (c). Palibe gulu lachilengedwe lomwe limapezeka m'chigawo choyeserera (T). |
| WopundukaMalipiro:
| Bandi yolamulira imalephera kuwonekera. Zotsatira za mayeso ena omwe sanatulutse bandi yowongolera nthawi yomwe yawerengedwa iyenera kutayidwa. Chonde onaninso njirayi ndikubwereza ndi mayeso atsopano. Ngati vutoli likupitilira, silimangogwiritsa ntchito zida nthawi yomweyo ndikulumikizana ndi wogulitsa kwanu. |
ZINDIKIRANI:
1. Kukula kwa mtundu mu dera loyeserera (T) kumatha kusiyanasiyana kutengera kutengera zinthu zomwe zikugwirizana ndi zomwe muli nazo. Koma zinthu zomwe zinthu sizingatsimikizidwe ndi mayeso oyenerera.
2. Kugwiritsa ntchito mawu osakwanira, njira yolakwika, kapena kuyeserera kopambana ndi zifukwa zake zomwe zingakhale zolephera kuwongolera band.
Kuwongolera kwapadera
■ Zowongolera zama procesdur zamkati zimaphatikizidwa pamayeso. Gulu lauda lomwe limawonekera m'chigawo chowongolera (c) limawonedwa ngati njira yotsimikizika yamkati. Imatsimikizira njira yokwanira yolondola komanso yolondola ya proceddiel.
■ Zowongolera za Procesdur zakunja zitha kuperekedwa (pofunsira kokha) m'makola kuti zitsimikizidwe kuti mayeserowa akugwira bwino ntchito. Komanso, zowongolera zitha kugwiritsidwa ntchito posonyeza magwiridwe antchito moyenera ndi woyeserera. Kuchita mayeso abwino kapena osavomerezeka, malizitsani magawo omwe ali mu gawo la mayeso omwe amayesa kuwongolera swab mofananabwino.
Zoperewera za mayeso
1. Palibe kumasulira kochuluka komwe kumayenera kupangidwa kutengera zotsatira zoyeserera.
2.Do osagwiritsa ntchito mayeso ngati thumba lake la aluminium kapena zisindikizo za thumba sililime.
3.a®Zotsatira Zoyeserera, ngakhale zikuwona kukhalapo kwa madzimadzi amadzimadzi, sapeza tsamba la chotupacho.
4.Ndi ndi mayeso onse ozindikira, zotsatira ziyenera kutanthauziridwa powala kwa zomwe wapeza.
5.Iti mitu ya fetal yachitika koma kutayikira kwamadzi a amniotity kwatha maola 12 musanachitike chifukwa cha ma rugina ndipo mayeso atha kupereka zotsatira zoyipa.
Makhalidwe Akugwirira Ntchito
Gome: Mphamvu®Chiyeso cha Phward vs. Mayeso ena otchuka
|
Kukhumudwa kwa Mwachibale: |
| Mtundu wina |
| ||
| + | - | Zonse | |||
| Cholimba®GARA Yesa | + | 63 | 3 | 66 | |
| - | 2 | 138 | 140 | ||
|
| 65 | 141 | 206 | ||
Chidwi chowunikira
Kuchuluka kotsika kwambiri kwa igbbp-1 mu zitsanzo zobwezeretsedwa ndi 12.5 μg / l.
Kusokoneza zinthu
Chisamaliro chiyenera kusokonezedwa kuti usaipitse wofunsira kapena wopanda zisungunuke ndi mafuta, sopo, mafuta ophera tizilombo, kapena mafuta. Mafuta kapena zonona amatha kusokoneza mayamwidwe omwe ali pachiwonetserocho. Sopo kapena opha tizilombo amatha kusokoneza antibody-antigen.
Zinthu zomwe zingachitike zimayesedwa mozama zomwe zingapezeke moyenerera ku Cervicovaginal secretions. Zinthu zotsatirazi sizinasokoneze poganiza mukamayesedwa m'magawo omwe awonetsedwa.
| Chinthu | Kolimbikira | Chinthu | Kolimbikira |
| Ampicillin | 1.47 mg / ml | Prostaglandin F2 | 0.033 mg / ml |
| Erythromycin | 0.272 mg / ml | Prostaglandin e2 | 0.033 mg / ml |
| Mkodzo wa amayi 3rr trimester | 5% (Vol) | Monistatr (miconazole) | 0,5 mg / ml |
| Oxytocin | 10 iu / ml | Carmino carmine | 0.232 mg / ml |
| Terbutaline | 3.59 mg / ml | Wamemicin | 0.849 mg / ml |
| Dexamethasone | 2.50 mg / ml | Beadiner gel | 10 mg / ml |
| Mgso4•7h2o | 1.49 mg / ml | Woyeretsa Betadineer | 10 mg / ml |
| Ritodrine | 0.33 mg / ml | K-yr odzola | 62.5 mg / ml |
| Dermidolr 2000 | 25.73 mg / ml |
Zolemba za mabuku
Erdemooglu ndi mungin t. Kutanthauza kuwunika kwa protekin-1 yopenda ma protekin-1 mu cervicovagination scretetions: Kufanizira ndi mayeso a nitrazine amayesedwa. ASCOCT Gynecol Spond (2004) 83: 622-626.
Kubota t ndi seachi h. Kuunikira kwa kukula kwa insulin, monga proctor protein-1 monga chida chofufuzira cha kusokonekera kwa nembanemba. J.Sttet gynecol rep (1998) 24: 411-417.
Rutanen et et al. Kuyika mayeso am'madzi mwachangu kwa insulin-ngati proctor protein-1 pakupezeka kwa fetal membranes. Chipatala Chim (1996) 253: 91-101.
Rutanen Em, Pekonen F, Karkkainen T. Kukhazikitsa kwa Insulin-ngati proctor protein-1 infretions: Kufanizira ndi rom-Check Immbral membral membral membral membral membral membral membral membral membral membral membral membral membral. Chipatala Chim (1993) 214: 73-81.
Zaikira za Zizindikiro
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| Nambala ya catalog |  | Kuchepetsa kutentha |
 | Konzani malangizo |
| Code |
 | Mu diagracic Diactic chipangizo chowonjezera |  | Gwiritsani ntchito |
 | Kupanga |  | Ili ndi zokwanira |
 | Osagwiritsanso ntchito |  | Woyimira wovomerezeka ku Europe |
 | CE yokhala ndi zida za IVD Medical Atsogoleri 98/79 / EC | ||
