Kugwiritsa Ntchito
Nyemba^®Adenovirus Flight Chipangizo Chachikulu (Ndondomeko) ndi zowoneka mwachanguimmunosAs for of the imyetsetsa kuzindikiritsa adnovirururururus mwa munthumapangidwe amwano. Izi zikugwiritsidwa ntchito kuti mugwiritse ntchito ngati thandizo pakuzindikira za adenovirus
matenda.

Chiyambi
Artuc adenoviruses, makamaka ad40 ndi Ad41, ndi choyambitsa m'mimbaMu ana ambiri omwe ali ndi vuto la diviro matenda, sekondikokha kwa clobiroses. Matenda opatsirana pachimake ndi chifukwa chachikulu cha imfaMu ana achichepere padziko lonse lapansi, makamaka m'maiko otukuka. AdenovirusTizilombo toyambitsa matenda tadzipatula padziko lonse lapansi, ndipo zimatha kuyambitsa matenda otsetserekaMu chaka cha ana ozungulira. Matenda amawoneka pafupipafupi mwa ana ocheperaNdili ndi zaka ziwiri, koma zapezeka mwa odwala azaka zonse.Kafukufuku akuwonetsa kuti adenoviruses amagwirizanitsidwa ndi 4-15% yonseZipatala zogonekedwa m'chipatala za gastroenteritis.

Kuzindikira komanso mofulumira komanso molondola kwa gastroenterititititititinis ndi kothandizaPokhazikitsa etiology ya gastroenteritis komanso kasamalidwe ka wodwala.Njira Zina Zomwe Zimachitika Monga Ma Microncopy (EM) ndiMa nucleic acid hybrirization ndiokwera mtengo komanso ntchito yambiri. Pozindikira zaKudzipatula kwa matenda a adenovirus, okwera mtengo komansoKuyesedwa kovuta kwambiri sikungakhale kofunikira.

Maganizo
Chida cha Adenovirus Flust Chaldert (ndowe) chimazindikira Adenoviruskudzera kutanthauzira kowoneka kwa chitukuko cha utotoMzere. Anti-Adenoviruriss antibodies ndi osinthika kudera lanembanemba. Poyesedwa, fanizo limagwira ndi anti-adennorurururiss antibodiesophatikizidwa ndi tinthu tating'onoting'ono ndikuwonetsa pampando wa mayeso a mayeso.Osakaniza kenako amasamukira mu nembanemba ndi capillary kuchitapo kanthundi ma reagents pa nembanemba. Ngati pali okwanira adenovirus mu fanizoli, aGulu lauda lauda lidzapangika kudera la nembanemba. Kukhalapo kwa iziGulu lauda limawonetsa zotsatira zabwino, pomwe kulibe kuwonetsazotsatira. Maonekedwe a gulu lauda padera lolamulira limakhala ngatiprocesdural yowongolera, kuwonetsa kuti kuchuluka kwa fanizoli kwakhalaZowonjezera ndi membrane akuwoneka kuti wachitika.

Machitidwe
Bweretsani ziyeso, zoyerekeza, buffer ndi / kapena kuwongolera kutentha kwa chipinda(15-30 ° C) musanagwiritse ntchito.
1. Kutolere pa Centern ndi Pre-Pre-chithandizo:
1) Gwiritsani ntchito zotsekemera zoyera, zowuma pazokambirana. Zotsatira zabwino zidzakhalazopezeka ngati lingaliro limachitika mkati mwa maola 6 mutasonkhanitsa.
2) Zolinga zolimba: UNSCERE ndikuchotsa wofunsira chubu. KhalaSamalani kuti musapatse kapena kubwereza yankho kuchokera ku chubu. Sungani zonenapoika wofunsira amagwira matsamba atatu osiyanasiyana andowe zoti zisonkhanitse pafupifupi 50 mg ya ndowe (zofanana ndi 1/4 za pea).Kwa zoyerekeza zamadzimadzi: Gwirani pipette mophweka, aspirate dricalzonena, kenako ndikusamutsa madontho awiri (pafupifupi 80 μl) kulowaChovala chosungira chosungira chomwe chili ndi buffer.
3) Sinthani wofunsira kumbuyo mu chubu ndikuyika kapu mwamphamvu. KhalaSamalani kuti musaswe nsonga ya chubu.
4) Gwedezani chubu chosungira chosungiramo mwamphamvu kusakaniza fanizo ndithunthu la m'zigawo. Zoyeserera zokonzedwa mu chubu cholumikiziraitha kusungidwa kwa miyezi 6 pa -20 ° C ngati osayesedwa mkati mwa ola limodziKukonzekera.

2. Kuyesa
1) Chotsani mayesowo m'thumba lake losindikizidwa, ndikuyikamawonekedwe oyera. Lambulani kuyesa kwa wodwala kapena kuwongolerachizindikiritso. Zotsatira zabwino, chigamulo chikuyenera kuchitidwa mkati mwa umodziora.
2) Kugwiritsa ntchito chidutswa cha pepala, ndikuphwanya nsonga ya chubu. Gwirachubu chokhazikika komanso choponyera madontho atatu a yankho mu fanizoli(S) za chipangizo choyeserera.Pewani kutchera ma thovu a mpweya mu chinsalu (s), ndipo osawonjezera
yankho lililonse pazenera.Pamene mayeso amayamba kugwira ntchito, mtundu umasamuka ku nembanemba.

3. Yembekezerani gulu lauda kuti liziwoneka. Zotsatira ziyenera kuwerengedwa pa 10Mphindi. Osatanthauzira zotsatira pambuyo 20 mphindi.

Zindikirani:Ngati fanizo silimasamukira chifukwa cha kukhalapo kwa tinthu tating'onoting'ono, centrifugeZoyimira zobwezeretsedwa zomwe zili mu buffer buffer vial. Sungani 100 μl yaSupernatant, dinani mu chinsalu cha chinthu choyesera (s) cha chipangizo choyesera chatsopano ndikuyambanso, kutsatira malangizo omwe tafotokozera pamwambapa.

Chipangizo