Chipangizo champhamvu champhamvu cha SARS-Cov-2 Antigen First
Kugwiritsa Ntchito
Chida champhamvu champhamvu ® cha SARS-Cov-2 Antigen Flient Filly Technolography Technology kuti adziwe za Sars- Cov-2 Nucleocapsid antigen mu malovu aanthu. Kuyezetsa kumeneku ndi kugwiritsidwa ntchito kamodzi kokha ndikukonzekera kuyesedwa kwa sei. Ndikulimbikitsidwa kugwiritsa ntchito kuyesedwa uku mkati mwa masiku 7 a chizindikiro kumachitika kumathandizidwa ndi kuwunika kwamankhwala.
Chiyambi
The New Coronacirses ali a 0 gerus. Covid-19 ndi matenda opatsirana pachimake. Anthu nthawi zambiri amabwera. Pakadali pano, odwala omwe ali ndi matenda a Coronavirus ndiye gwero lalikulu la matenda opitsidwa ndi kupenda kwa epidemogical komwe kulipo, nthawi ya makulitsidwe ndi masiku 1 mpaka 7. Ziwonetsero zazikulu zimaphatikizapo kutentha thupi, kutopa komanso chifuwa chowuma. Kugontha kwa mphuno, mphuno zowotchera, zilonda zapakhosi, myalgia ndi kutsekula m'mimba zimapezeka pamilandu yochepa.
Maganizo
Chida champhamvu champhamvu® cha SARS-Cov-2 Antigen mayeso a immunoromatographict, ndipo ma sava amapita patsogolo pa capillary. Ngati zitsanzo zili ndi SARS-Cov-2 N Furtenin Antigen.it imadziwika ndikumangidwa ndi ma antibodies omwe amalembedwa pamtunda wa latex kuti apange zovuta za latex. Pamene mphamvu yopangidwa ndi itric acid acid acid acid, kuti muzindikire ma antibodies okhala ndi ma antibodies okhala ndikupanga mzere wa fuchsia disc (T-Shev-2 Antigen wabwino; Ngati chingwe sichikuwonetsa mtundu, ndizowopsa. Chingwe china pa nitric acid nembanemba zimadzaza ndi ma antibodiidin a ma antibodines ngati mzere wowongolera (C) kuwonetsa kuyeserera koyenera.
KUSAMALITSA
• Kityi ili chifukwa cha matenda a Vitro ogwiritsa ntchito okha.
• Izi zitha kuperekedwa ndi azachipatala kapena osagwiritsa ntchito zamankhwala potsatira malangizo ogwiritsira ntchito.
Werengani malangizo mosamala musanayesere mayeso.
• Izi sizili ndi zida zilizonse zamunthu.
• Musagwiritse ntchito zomwe zili mu Tot tsiku lotha ntchito.
• Sanjani zonena zonse zomwe zingakhale zovuta.
• Osamatsuka pakamwa ndikusuta fodya kapena kudya kwinaku.
• Valani magolovesi mu njira yonse.
Kusunga ndi kukhazikika
Zimbudzi zosindikizidwa mu Kit zitha kusungidwa pakati pa 2-30 ° ° C kwa nthawi ya alumali monga tasonyezedwa pa thumba.
Kutolera mwachidule ndi kusungidwa
Makina abwino kwambiri a sava ayenera kusonkhanitsidwa m'mawa mutangodzuka. Osamadya kapena kumwa chilichonse kwa mphindi 30 musanatole saiva wanu. Chitani izi musanadye khofi, kudya chakudya cham'mawa, kapena kutsuka mano anu - kapena dikirani mpaka simunadye chilichonse mu mphindi 30 zapitazo.
Machitidwe
Bweretsani ziyeso kuti muthe kutentha kwa chipinda (15-30 ° C) musanagwiritse ntchito.
Gawo 1:
Tsegulani thumba, tengani chipangizo choyeserera, tsegulani chivundikiro cha chipangizochi.
2:
• Gwiritsani ntchito kaseti, ikani malovu a malovu omatira pansi pa lilime, khazikitsani ndodo ndi lilime lokwanira masekondi 120.
• Sungani chipangizocho molunjika ndi lolani kuti mchere wamadzimadzi azisunthira m'mwamba mpaka pamzere wa C, kenako kwezani kapu.
• Ikani chipangizocho molunjika pa ntchito.
Gawo 3:
Werengani nthawi ndipo werengani kupezeka kwa Rutheh mphindi 15 pambuyo pake.
Ponyani zinyalala mu chidebe cha biohazi.
Kuwongolera kwapadera
Zowongolera zamkati za procesdul zimaphatikizidwa poyesedwa. Gulu la buluu lomwe limawonekera kudera la Control (c) limawonedwa ngati njira yamkati. Imatsimikizira njira yokwanira yolondola komanso yolondola ya proceddiel.
Kuchepetsa Mayeso
1. Chigawocho chilingafuna kugwiritsa ntchito kuzindikiritsa kwa SARS-Cov-2 a Antigens ochokera ku malovu.
2. Kuyeza kumeneku kumazindikira zonse zothandiza (moyo) komanso zomwe sizikugwiritsa ntchito-cov-2. Kuyeserera koyeserera kumadalira kuchuluka kwa kachilombo (antigen) mu zitsanzo ndipo mwina sangaphatikize ndi zikhalidwe zomwe zimachitika ndi zotsatirazi zomwezo.
3. Kuyeserera kolakwika kumatha kuchitika ngati mulingo wa antigen mu zitsanzo zomwe zili pansi pa mayeso a mayeso kapena ngati chitsanzocho chidatengedwa kapena kunyamula molakwika.
4. Kulephera kutsatira njira yoyesera kungasokoneze mayesero oyeserera ndi / kapena kusokoneza zotsatira zoyeserera.
5. Chidacho ndi chongoyang'ana kokha. Zotsatira zoyipa sizimagwiritsa ntchito matenda a SAR-Cov-2 ndipo munthu amene sapusilana. Ngati zizindikiro zilipo, pezani nthawi yomweyo kuyesedwa kwina.
6. Zotsatira zoyesedwa ziyenera kuphatikizidwa ndi mbiri yamankhwala, deta ya miliri, ndi deta ina ya chipatala chowunikira wodwalayo.
7. Zotsatira zabwino sizikunena za matenda omwe ali ndi tizilombo toyambitsa matendawa ndipo sangadziwe ngati munthu wagona.
8. Zotsatira zoyipa sizikupangidwira kuti zigwirizane ndi ma virus kapena bakiteriya.
9. Zotsatira zoyipa kuchokera kwa odwala omwe ali ndi chizindikiro, ziyenera kuthandizidwa ngati kutsimikizika ndi kazembe wa FDA yovomerezeka, ngati kuli kofunikira, pochita mankhwala osokoneza bongo, kuphatikiza kuwongolera matenda.
. Ogwiritsa ntchito ayenera kuyesera zoyesayesa mwachangu pambuyo pa zokambirana.
. m'munsi chifukwa cha njira zake.
12. Mfundo zabwino komanso zosalosera zimadalira kwambiri mitengo ya anthu.
Zotsatira zabwino zimayimira zolakwa zabodza nthawi yayitali / palibe ntchito yoyeserera.
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15. Kuchuluka kwa antigen munkhosa kumachepetsa monga nthawi yayitali ya matenda amawonjezeka.
Zolingana zomwe zimasungidwa pambuyo pa tsiku 7 za matenda zimatheka kuti zikhale zolimbikitsa poyerekeza ndi A RCR Calr.
Kukhumba kwa mayeso atatha masiku asanu ndi awiri a kuyamba kwa zizindikiro zakhala zikudziwika kuti kuchepa poyerekeza ndi A RCR akuganiza.
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17. Kuyesa pafupipafupi ndikofunikira kuti muwonjezere matenda a Covid-19.
. B.1.351; B.1.2; B.1.1.2.28; B.1.67; B.1.1.529.
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Pangano Lililonse: (Papa) = 98.02% (93.03% ~ 99.76%) *
Mgwirizano Wosalimbikitsa: (NPA) = 100% (98.23% ~ 100%) *
Mulingo Wocheperako = 98.76%
* 95% yolimba mtima
Magwiridwe antchito
a) malire a chizindikiritso (LoD):
Malire opezeka (LoD) yoyesedwayo idatsimikizika pogwiritsa ntchito machepetse a SARS-Cov-2. Ndikukonzekera coronavirus okhudzana ndi a Corsavirus-2 (SARS-COV-2), kudzipatula ku China CDC, yomwe yasinthidwa ndi β-equlalactone. Zinthuzo zidaperekedwa mozizira pa tcid50wa 5.00 x105/ ml.
Kuti mudziwe SARS-Cov-2 kuti muwonetsetse lingaliro mukamagwiritsa ntchito malovu mwachindunji. Mu phunziroli pafupifupi 50μL la kuchepa kwa kachilomboka chidaphikidwa ndi malovu osasangalatsa.
Lod adatsimikizika m'masitepe atatu:
1. Zolemba
Masikono 10 a virus omwe adalipo omwe adapangidwa ndi malovu osamukira ndikukonzedwa pa kafukufuku aliyense monga tafotokozera pamwambapa. Kuchepetsa uku kunayesedwa mtsogolo. Kuzindikira kuwonetsa 3 mwa zinthu zitatu zomwe zimasankhidwa kuti Lod apeze.
2. LOD Generance kupeza
Zisanu (5) zochezera zochulukirapo zidapangidwa ndi tcid50wa 5.00 x102/ ml ndende mu sava zoyipa zomwe zakonzedwa pophunzira monga tafotokozera pamwambapa. Kuchepetsa uku kunayesedwa mtsogolo. Kuzindikira kuwonetsa 3 mwa zinthu zitatu zomwe zasankhidwa kuti itsimikizidwe.
3. Lod Chitsimikizo
Kuchuluka kwa tcid50wa 2.50 x102/ ML Dincetion idayesedwa kwa zotsatira makumi awiri (20). Zotsatira zisanu ndi zinayi (19) za zotsatira makumi awiri (20) zinali zabwino.
Pomaliza:
Kutengera kuyesa uku kunatsimikiziridwa kuti:
LOD: TCID502.50 x102/ ml
b) Mtanda-Upata:
Kutalika kwa Chida cha AlcesPep® Systery SAR-COV-2 Antigen Kuyesa Kuyesa Microorganisms (10 ⁶ cfu / ml), matruble (10⁵ pl) ndi ma matrix omwe angachite Chida cha Chipangizo cha Dongosolo la SAR-Cov-2 antigen kuyeserera mwachangu.
Chiwalo chilichonse komanso kachilomboka chidayesedwa m'mbuyo. Kutengera ndi zomwe zapangidwa ndi phunziroli, chipangizo cha System® cha SARS-COV-2 Antigen Stud sichimadutsa - zomwe zimakhudzana ndi zolengedwa kapena ma virus omwe adayesedwa.
c) Kusokoneza Zinthu:
Zinthu zomwe zingatheke kusokoneza zinthu zamphamvu zamphamvu ®. Chilichonse chidayesedwa m'mbuyo. Kutengera ndi zomwe zapangidwa ndi phunziroli, SAVSSEP® SARS-CAV-2 Antigen Flience imasokoneza zinthu zomwe zimayesedwa.
d) Zowopsa
Kukhazikika kwambiri kwa SARD-STRACTIV-COV-2 yomwe ilipo (TCID50wa 5.00 x 105/ ml) adayesedwa. Panalibe chowonera.
