New Coronavirus (SARS-COV-2) ACHINSX Real-nthawi
Chovuta kwambiri cha PCR, chokonzekera PCR chikupezeka mu mtundu wa lyophilized (njira yowuma) yosungirako kwa nthawi yayitali. Kityi ikhoza kunyamulidwa ndikusungidwa mu kutentha kwa firiji ndipo imakhazikika chaka chimodzi. Chingwe chilichonse cha premix chili ndi ma reagents onse omwe amafunikira pa PCR Atalikidwe, kuphatikiza kumbuyo-transcriptase, primes, ndi ma dnts. Zimangofunika kuwonjezera madzi 13,000 osungunuka ndi 5ul yotulutsa RNA template, ndiye kuti imatha kuthamangitsidwa ndikukukulitsidwa pazida za PCR.
Makina a QPCR ayenera kukwaniritsa zofunikira zotsatirazi:
1.
2.
| Ngalande | Chisangalalo (NM) | Emations (nm) | Utoto wosakhazikika |
| 1. | 470 | 525 | Banja, sybri green i |
| 2 | 523 | 564 | Vic, Hex, Tet, Joe |
| 3. | 571 | 621 | Rox, Texas-Red |
| 4 | 630 | 670 | Cy5 |
PCR-Platifomu:
7500taal pcr system, biorad cf96, ic icycley iq yes-nthawi zonse zowunikira, stratne mx3000p, MX3005P
Chovuta cha mayendedwe ozizira a coronavirus nuclenaviriss acid
Pamene ovomerezeka a nyukiliya a acid amatengedwa mtunda wautali, (-20 ± 5) Kuti muwonetsetse kuti kutentha kumafika pamlingo, ma kilogalamu angapo a madzi oundana amafunikira pabokosi lililonse la ma ecleic acid kuyesa kopitilira 50g, koma kumatha kupitilira masiku awiri kapena atatu. Pa malingaliro azochita zamakampani, kulemera kwenikweni kwa opanga ndi ochepera 10% (kapena ocheperako kuposa mtengo wake). Mafuta ambiri amachokera ku ayezi wouma, matope a madzi ndi mabokosi am'matambo, kotero mtengo wake umakhala wokwera kwambiri.
Mu Marichi 2020, Covid-19 adayamba kugwera kunja, ndipo kufunikira kwa coronavirus nyukiliya a acid kumachitika kwambiri. Ngakhale kuti pali mtengo wokwera potumiza ma reagents mu unyolo wozizira, opanga ambiri amatha kuvomereza chifukwa cha kuchuluka kwakukulu komanso phindu lalitali.
Komabe, ndikusintha kwa zinthu zakunja kwa zinthu zovomerezeka za anti-mliri, komanso kukweza kwa dziko lapansi kwa anthu ndi kusatsimikizika komanso kusatsimikizika mu nthawi yoyendera yomwe yachitika ndi mayendedwe. Nthawi yowonjezera yoyendera (nthawi yoyendera pafupifupi theka la mwezi ndizofala kwambiri) zimabweretsa zolephera zodwala zomwe zimachitika kawirikawiri malonda. Izi zasautsa mabizinesi ambiri a acid omwe amasintha.
Maukadaulo a LyOpholired Offr adathandizira mayendedwe a Coronavirus nyukiliya a acid yotchuka padziko lonse lapansi
Makina a LyOpholid PCR amatha kunyamulidwa ndikusungidwa kutentha, zomwe sizingangochepetsa ndalama zoyendera, komanso kupewa mavuto abwino omwe amachitika chifukwa cha mayendedwe. Chifukwa chake, ldziiliza regiling ndiye njira yabwino kwambiri yothetsera vuto la mayendedwe kunja.
A Lyophlilization imaphatikizapo kuzizira kotheratu kukhala lolimba, kenako ndikugonjera ndikulekanitsa madzi m'madzi omwe ali ndi vuto la vacuum. Solu yopukuta imakhalabe mumtsuko womwewo ndi zomwezi. Poyerekeza ndi zowongolera zamadzimadzi, zomwe zimadziwika bwino kwambiri za vasonzavirus nyukiliya a acid zopangidwa ndi liwiro lopangidwa ndi ma likeming bio ali ndi machitidwe awa:
Kukhazikika Kwambiri Kwambiri:Itha kukhala ndi Chithandizo cha 56 ℃ kwa masiku 60, ndi morphology ndi magwiridwe antchito osasinthika.
Kutentha kwabwinobwino kwa kutentha ndi mayendedwe:Palibe chifukwa cha unyolo wozizira, palibe chifukwa chosungira kutentha kochepa pamaso pa kusala, kumasula bwino malo ozizira ozizira.
Kukonzekera Kugwiritsa Ntchito:Kudzikuza kwa zinthu zonse, osafunikira kusinthidwa kwa dongosolo, kupewa kutaya zinthu zomwe zimakhala ndi ma viloni apamwamba ngati enzyme.
Mitengo ya Mixxx mu chubu chimodzi:Cholinga cha chizindikilo chimaphimba chatsopano Coronavirus Orf1ab Gene, N Gene, S Geno kuti mupewe mtundu wa kachilomboka. Pofuna kuchepetsa zabodza zoyipa, rnase yamunthu p
