Fetal fibronectin mayeso achangu
Gwiritsani ntchito
Nyemba®Kuyesedwa kwa Promoromatophic kuyesedwa komwe kumapangitsa kuti agwiritsidwe ntchito kupezeka kwa fetal fibronectinactions. Kukhalapo kwa fetal fibronectin infretions (masabata atatu aliwonse, masiku 34, masiku 6 a bestation alizogwirizana ndi chiopsezo chokwezeka choperekera kwa presterm.
Chodumira
Kutumiza kwa Stenter Zizindikiro zakuwopseza kwa Stenter Preent zimaphatikizapo zophatikizira za uterine, kusintha kwa zotupa zamkati, kutaya magazi, zam'mbuyo, m'mimba, komanso kupsinjika. Kuzindikira Kuzindikira Kuzindikira Kuwonongeka kwa Stenter Njirazi zawonetsedwa kuti ndizochepa, monga miyeso yocheperako (<3 masentimita) ndi chiberekero) ndi chiberekero zimachitika nthawi zambiri ndipo sikuti zimapezeka kawirikawiri popereka chithandizo cha Stesterm. Ngakhale kuti zilembo zingapo za seramu zambiri zawunika, palibe amene adalandiridwa kwambiri pakugwiritsa ntchito.
Fetal Fibronectin (FFN), kuwongolera kwa fibronectomn, ndi zovuta kwambiri zomatira glycofot glycotein wokhala ndi ma daltoon apamwamba pafupifupi 500,000. Mattiuura ndi ogwira nawo ntchito afotokoza za antibody wotchedwa FDC-6, yomwe imazindikira mwachindunji III-Cs, dera lomwe likufotokoza za fetal. Kafukufuku wa immunohisthamical of platntae awonetsa kuti ffn ndiKukhazikika kwa matraclerlular a kuderali akufotokozerani mphambua mayunitsi a amayi ndi fetal mkati mwa chiberekero.
Fetal Fibronectin imatha kupezeka pachimake cha cervicovaginal cha amayi pa mimba pogwiritsa ntchito antibollodybadleasm. Fetal Fibronectin imakwezedwa m'chinsinsi cha cervicovaginal nthawi yayitali koma imatha kuchokera pathano milungu 22 mpaka 35. Kufunika kwa kupezeka kwake mu nyini kumayambiriro kwa miyezi yoyambirira ya mimba sikumveka. Komabe, zitha kuwonetsetsa kuti kuchuluka kwa chinsalu cha nsanja yowonjezera ndi placeda. Kuzindikira FFN ku Cervicovaginal infretions pakati pa masabata 22, masiku 34, kufalikira kwa masiku 34, kumachitika pakati pa masabata atatu, masiku 30 ku Asymptomatic amayi oyembekezera.
Maganizo
Nyemba®Kuyesedwa kwa FFN kumagwiritsa ntchito utoto immunoromatographict, ma capillary oyenda. Njira yoyesera imafuna kusungunuka kwa ffn kuchokera ku swab ya swab posakaniza swab mumtundu wamtunduwu. Kenako buffer yosakanikirana imawonjezeredwa ku Cassette yamayeso yoyeserera bwino ndipo osakaniza amasamukira kukhoma. Ngati FFN ilipo mu chitsanzo, chidzapanga zovuta ndi antibody anti-anti oyamba omwe amaphatikizidwa ndi tinthu tating'ono. Mavuto amphamvu adzamangidwa ndi antibody wachiwiri womenyedwa ndi Nitrocellulose nembanemba. Maonekedwe a mzere woyeserera wowoneka komanso mzere wowongolera uziwonetsa zotsatira zabwino.
Zigawo zikuluzikulu
| 20 Payekha pankhaZida za Ed | Chipangizo chilichonse chimakhala ndi chingwe chokhala ndi conjuban conjugunk komanso zopangidwa ndi zisanachitike pamalo ofanana. |
| 2KutulutsaBuffer vial | 0.1 m phosphate yolumikizidwa saline (PBS) ndi 0.02% sodium azide. |
| 1 yodzitchinjiriza swab (pofunsira) | Khalani ndi FFN ndi sodium azide. Chifukwa chowongolera zakunja. |
| 1 zoyipa swab (pofunsira) | Osakhala ndi FFN. Chifukwa chowongolera zakunja. |
| 20 Chigawo cha madzi | Zogwiritsa ntchito zowerengera. |
| 1 Kumanga | Ikani ndodo yolumikizira ndi machubu. |
| 1 Kuyika kwa phukusi | Kulangizidwa. |
Zida zofunika koma sizinaperekedwe
| Ikanthawi | Kugwiritsa ntchito nthawi. |
KUSAMALITSA
■ kwa akatswiri mu diactro wozindikira pokhapokha.
Osagwiritsa ntchito tsiku lotha ntchito lomwe lawonetsedwa pa phukusi. Osagwiritsa ntchito mayeso ngati thumba lake lowonongeka lawonongeka. Osagwiritsanso ntchito mayeso.
■ Towa ili ndi zinthu za zoyambira zanyama. Chidziwitso chotsimikizika cha chiyambi ndi / kapena chimbudzi cha nyama sizitanthauza kuti kusakhalapo kwa othandizira pathogenic. Chifukwa chake, ndikulimbikitsidwa kuti zinthu izi zimathandizidwa ngati mwazotheka, ndikuzisamalira mosamala (sizikuphatikiza kapena zotupa).
Musapewe kuipitsidwa ndi zoyeserera za zoyeserera pogwiritsa ntchito chidebe chatsopano cha fanizo lililonse.
Werengani njira yonse musanayambe kuyesedwa kulikonse.
Osamadya, kumwa kapena kusuta m'dera lomwe zoyerekeza ndi ma kits zimayendetsedwa. Samalani zonena zawo ngati ali ndi opatsirana opatsirana. Kuchita mosamala motsutsana ndi zoopsa zomwe zimachitika munthawi yonseyi ndikutsatira njira zoyenera zomwe zimatheka. Valani zovala zoteteza monga malaya a labotale, magolovesi otayika ndi kutetezedwa ndi malingaliro pomwe malingaliro aja aja.
Osasokoneza kapena kusakaniza regents kuchokera ku maenje osiyanasiyana. Osasakaniza yankho la botolo.
Chinyezi ndi kutentha zimatha kuvuta kwambiri.
Nthawi yomwe bungwe lagamulo limamalizidwa, pezani ma swab mosamala atatha kuwaletsetsa pa 121 ° C kwa mphindi 20. Kapenanso, amatha kuthandizidwa ndi 0,5% sodium hypochloride (kapena bulichi) kwa ola limodzi atatayidwa. Zipangizo zoyeserera zomwe zogwiritsidwa ntchito zimayenera kutayidwa molingana ndi kwanuko, boma ndi / kapena feduro.
Musagwiritse ntchito mabungwe a cytology ndi odwala apakati.
Kusunga ndi kukhazikika
■ Kitt iyenera kusungidwa pa 2-30 ° C mpaka tsiku lomaliza losindikizidwa ku thumba losindikizidwa.
■ Mayeso ayenera kukhala mu thumba losindikizidwa mpaka kugwiritsira ntchito.
Osasintha.
Mayankho ayenera kutengedwa kuti ateteze zinthu zomwe zimapezeka mu zoipitsidwa. Osagwiritsa ntchito ngati pali umboni wa kuipitsidwa kapena mpweya wabwino. Kuipitsa kwachilengedwe kwa kufalitsa zida, zotengera kapena zoyeserera zimatha kubweretsa zotsatira zabodza.
Pecimen Collec ndikusunga
■ gwiritsani ntchito ducron yokha kapena rayn zidagunda swab shabs ndi ma pulasitiki. Ndikulimbikitsa kugwiritsa ntchito swab yomwe imapereka ndi opanga (Swabs sanakhalepo pachiwonetserochi, kuti mudziwe zambiri, chonde lemberani wopanga kapena kugulitsa kwapafupi, nambala ya cataloge ndi 207000). Ma swabs ochokera kwa othandizira ena sanavomerezedwe. Swabs ndi upangiri wa thonje kapena ma shargets a matabwa sakulimbikitsidwa.
Makonda a Cervicovaginal amapezeka kuchokera ku nkumacha ndi nyini ya nyini. Njira yosungirayo idapangidwa kuti ikhale yodekha. Kusonkhanitsa kwamphamvu kapena mwamphamvu, zofala kwa zikhalidwe zamatsenga, sizofunikira. Panthawi yoyeserera, musanayambe kuwunika kapena kusanthula kwa cervix kapena yisiti ya wofunsira, mopepuka mopepuka mpaka kumapeto kwa maliseche 10 kuti atenge ma servinal a cervicovaginal. Kuyesa kwotsatira kuti akhumudwitse thumba la Lothandizira kungachititse kuyesa mayeso. Chotsani wofunsirayo ndikuyesera mayeso omwe ali pansipa.
■ Ikani swab kupita ku chubu cha madzi, ngati mayesowo atha kuthamanga nthawi yomweyo. Ngati kuyezetsa kumeneku sikungatheke, zitsanzo zodwala ziyenera kuyikidwa mu chubu chowuma chosungira kapena kunyamula. Masabata amatha kusungidwa kwa maola 24 firiji (15-30 ° C) kapena sabata 1 pa 4 ° pa -20 ° CO. Zojambula zonse ziyenera kuloledwa kufikira kutentha kwa chipinda cha 15-30 ° C musanayesedwe.
Machitidwe
Bweretsani mayesero, zoyerekeza, buffer ndi / kapena kuwongolera kutentha kwa chipinda (15-30 ° C) musanagwiritse ntchito.
Ikani chubu choyera choyera m'malo ophatikizidwa. Onjezani 1ML ya kuchotsera kubowola ku chubu cha madzi.
Ikani spimen swab mu chubu. Sakanizani mwamphamvu yankho pozungulira swab mwamphamvu kumbali ya chubu katatu (pomwe kumamizidwa). Zotsatira zabwino zimapezeka pomwe fanizoli likusakanizidwa mwamphamvu mu yankho.
Werengani madzi ambiri momwe mungathere kuchokera ku swab poyang'ana mbali ya chubu chosinthika monga swab imachotsedwa. Osachepera 1/2 mwa zitsanzo zothetsera buffer ziyenera kukhalabe mu chubu chosamukira ku capillary chokwanira kuti chichitike. Ikani chipewacho pa chubu chotuluka.
Kutaya swab mu chidebe choyenera cha bioharkous.
■ Zosintha zomwe zimachotsedwa zimatha kusunga kutentha kwa mphindi 60 osakhudza chifukwa cha mayeso.
Kuchotsa mayesowo m'thumba lake losindikizidwa, ndikuyika pa oyera pamtunda. Lembani chipangizocho ndi chizindikiritso kapena chowongolera. Kuti mupeze zotsatira zabwino, chigamulo chikuyenera kuchitidwa mkati mwa ola limodzi.
Onjezani madontho atatu (pafupifupi 100 μl) ya zitsanzo zosinthidwa kuchokera ku chubu cha madzi opita ku Samsette yoyeserera.
Pewani kutchera ma thovu a mpweya mu chinsalu (s), ndipo musataye yankho lililonse pazenera.
Mayeso akayamba kugwira ntchito, mudzaona mtunduwo ukusunthira kudutsa nembanemba.
■ Yembekezerani gulu lazithunzi. Zotsatira zake ziyenera kuwerengedwa kwa mphindi 5. Osatanthauzira zotsatira pambuyo pa mphindi 5.
Tayani machubu oyeserera ndi ma cassette oyenera mu chidebe choyenera cha bioankhoous.
Ntergretis ya zotsatira
| WosaipidwaMalipiro:
| Magulu awiri achikuda amawonekera pa nembanemba. Bandi imodzi imawonekera kudera la Control (c) ndi gulu lina limapezeka m'chigawo (T). |
| WosavomelaMalipiro:
| Gulu limodzi lokha lamtundu limapezeka m'chigawo chowongolera (c). Palibe gulu lachilengedwe lomwe limapezeka m'chigawo choyeserera (T). |
| WopundukaMalipiro:
| Bandi yolamulira imalephera kuwonekera. Zotsatira za mayeso ena omwe sanatulutse bandi yowongolera nthawi yomwe yawerengedwa iyenera kutayidwa. Chonde onaninso njirayi ndikubwereza ndi mayeso atsopano. Ngati vutoli likupitilira, silimangogwiritsa ntchito zida nthawi yomweyo ndikulumikizana ndi wogulitsa kwanu. |
ZINDIKIRANI:
1. Kukula kwa mtundu mu dera loyeserera (T) kumatha kusiyanasiyana kutengera kutengera zinthu zomwe zikugwirizana ndi zomwe muli nazo. Koma zinthu zomwe zinthu sizingatsimikizidwe ndi mayeso oyenerera.
2. Kugwiritsa ntchito mawu osakwanira, njira yolakwika, kapena kuyeserera kopambana ndi zifukwa zake zomwe zingakhale zolephera kuwongolera band.
Kuwongolera kwapadera
■ Zowongolera zama procesdur zamkati zimaphatikizidwa pamayeso. Gulu lauda lomwe limawonekera m'chigawo chowongolera (c) limawonedwa ngati njira yotsimikizika yamkati. Imatsimikizira njira yokwanira yolondola komanso yolondola ya proceddiel.
■ Zowongolera za Procesdur zakunja zitha kuperekedwa (pofunsira kokha) m'makola kuti zitsimikizidwe kuti mayeserowa akugwira bwino ntchito. Komanso, zowongolera zitha kugwiritsidwa ntchito posonyeza magwiridwe antchito moyenera ndi woyeserera. Kuchita mayeso abwino kapena osavomerezeka, malizitsani magawo omwe ali mu gawo la mayeso omwe amayesa kuwongolera swab mofananabwino.
Zoperewera za mayeso
1. Udindowu ukhoza kugwiritsidwa ntchito kungodziwa kwa fetal fibronectin mu cervicovaginal secretions.
2. Zotsatira zoyeserera ziyenera kugwiritsidwa ntchito molumikizana ndi zidziwitso zina zamankhwala ndi labotale za kasamalidwe koleza mtima.
3. Zolingalira ziyenera kupezeka musanayambe kuwunika kwa digito kapena kupukusa kwa khomo lachiberekero. Kumanja za khomo lachiberekero kumatha kubweretsa zotsatira zabwino.
4. Zolingalira siziyenera kusonkhanitsidwa ngati wodwalayo wagonana mkati mwa maola 24 kuti athetse zotsatira zabwino.
5. Odwala omwe amakayikiridwa kapena odziwika kale, placenta previa, kapena magazi a ukazi kapena akuluakulu kapena akuluakulu sayenera kuyesedwa.
6. Odwala omwe ali ndi Cerclage sayenera kuyesedwa.
7.. Machitidwe amphamvu amphamvu®Kuyesedwa kwa FFN kumakhazikitsidwa pa maphunziro mwa akazi omwe ali ndi ma gestation. Kugwirira sikunatsimikizidwe kwa odwala omwe ali ndi maukwati angapo, mwachitsanzo, mapasa.
8. Mphamvu®Kuyesedwa kwa FFN sikunapangidwe kuti kuchitidwa pamaso pa kupasuka kwa amniotic ndi kuphedwa kwa Amniotic membranes ayenera kutetezedwa asanayambe mayeso.
Makhalidwe Akugwirira Ntchito
Gome: Mphamvu mwamphamvu vfn mayeso vs.
| Kukhumudwa kwa Mwachibale: 97.96% (89.13% -99.95%) * KULAMBIRA: 98.73% (95.50% --99.85%) * Pangano lonse: 98.55% (95.82% -99.70%) * * 95% yolimba mtima |
| Mtundu wina |
| ||
| + | - | Zonse | |||
| Cholimba®fFn Yesa | + | 48 | 2 | 50 | |
| - | 1 | 156 | 157 | ||
|
| 49 | 158 | 207 | ||
Chidwi chowunikira
Kuchuluka kotsika kwambiri kwa ffn mu sample yotengedwa ndi 50μg / l.
Mwamuna ndi azimayi okwezeka, milingo yokwezeka (≥ 0.050 μg / ml) (masiku 10-7) chotolera zitsanzo. Mwa azimayi a asymptotictictictictic, milingo yokwezeka pakati pa masabata 22, masiku 30 ndi masabata 30, masiku 6 akuwonetsa chiopsezo chowonjezereka chokamba mu ≤ 34, masiku 6 a bere. Kudula kwa 50 μg / l ffn kunakhazikitsidwa mu kafukufuku wa pakompyuta komwe kumachitika kuti awone kuyanjana pakati pa fetal fibronectin kufotokoza.
Kusokoneza zinthu
Chisamaliro chiyenera kusokonezedwa kuti usaipitse wofunsira kapena wopanda zisungunuke ndi mafuta, sopo, mafuta ophera tizilombo, kapena mafuta. Mafuta kapena zonona amatha kusokoneza mayamwidwe omwe ali pachiwonetserocho. Sopo kapena opha tizilombo amatha kusokoneza antibody-antigen.
Zinthu zomwe zingachitike zimayesedwa mozama zomwe zingapezeke moyenerera ku Cervicovaginal secretions. Zinthu zotsatirazi sizinasokoneze poganiza mukamayesedwa m'magawo omwe awonetsedwa.
| Chinthu | Kolimbikira | Chinthu | Kolimbikira |
| Ampicillin | 1.47 mg / ml | Prostaglandin F2 | a0.033 mg / ml |
| Erythromycin | 0.272 mg / ml | Prostaglandin e2 | 0.033 mg / ml |
| Mkodzo wa amayi 3rr trimester | 5% (Vol) | Monistatr (miconazole) | 0,5 mg / ml |
| Oxytocin | 10 iu / ml | Carmino carmine | 0.232 mg / ml |
| Terbutaline | 3.59 mg / ml | Wamemicin | 0.849 mg / ml |
| Dexamethasone | 2.50 mg / ml | Beadiner gel | 10 mg / ml |
| Mgso4•7h2o | 1.49 mg / ml | Woyeretsa Betadineer | 10 mg / ml |
| Ritodrine | 0.33 mg / ml | K-yr odzola | 62.5 mg / ml |
| Dermidolr 2000 | 25.73 mg / ml |
Zolemba za mabuku
1. American koleji ya obstetricians ndi akatswiri azachipatala. Ntchito yolerera. Tebulonilletin, nambala 133, Okutobala 1989.
2. RASY RK, Regnick R. amayi ndi fetal, mfundo ndi chizolowezi. Philadelphia: ma saunder; 1989.
3. Centry RK, Merkatz Ir. Kupewa kubadwa kwa preete: Malingaliro azachipatala. Syschet gynecol 1990; pfuena 1): 2s-4s.
4. Morrison JC. Kubadwa kwa Pretem: Chithunzi Chofunika Kuthekera. Syschet gynecol 1990; prel 1): 5s-12s.
5. Lockwood CJ, Senyai AE, disthe mr, Casal DC, et al. Fetal fibronectin mu zinsinsi komanso zamkati ngati zolosera za kufalitsa kwa Syretem. New Engl J Med 1991; 325: 669-74.
Zaikira za Zizindikiro
|
| Nambala ya catalog |  | Kuchepetsa kutentha |
 | Konzani malangizo |
| Code |
 | Mu diagracic Diactic chipangizo chowonjezera |  | Gwiritsani ntchito |
 | Kupanga |  | Ili ndi zokwanira |
 | Osagwiritsanso ntchito |  | Woyimira wovomerezeka ku Europe |
 | CE yokhala ndi zida za IVD Medical Atsogoleri 98/79 / EC | ||
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FinalStep® fetal Fibronectin First Diply chipangizo choyesera
Kutumiza kwa Stenter Zizindikiro zakuwopseza kwa Stenter Preent zimaphatikizapo zophatikizira za uterine, kusintha kwa zotupa zamkati, kutaya magazi, zam'mbuyo, m'mimba, komanso kupsinjika. Kuzindikira Kuzindikira Kuzindikira Kuwonongeka kwa Stenter
Amphamvu amphamvu a Fibronectin Fibronectin mayeso a Immunoromatographicd kuti agwiritsidwe ntchito kuti athe kupezeka kwa fetal fibronectinations mu cervicovinals ndi mawonekedwe otsatirawa:
Yosavuta kugwiritsa ntchito:Njira imodzi yoyeserera
Mwachangu:mphindi 10 zokha zomwe zimafunikira panthawi yomweyo
Zaulere:zipatala zosafunikira kapena makonzedwe azachipatala angachite mayeso awa
Kupulumutsidwa:kutentha kwa chipinda (2 ℃ -30 ℃)
